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4 2 2 1 recombinant floe1 cloning  (Addgene inc)


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    Addgene inc 4 2 2 1 recombinant floe1 cloning
    4 2 2 1 Recombinant Floe1 Cloning, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/4+2+2+1+recombinant+floe1+cloning/pGL3-Basic-9xSeq2-Luc+(Plasmid+%2342214)/flores_eduardo__2025__conformational_ensembles_of_intrinsically_disordered_regions_as_modulators_of_transcriptional_activity-1572-0-23
    Average 93 stars, based on 1 article reviews
    4 2 2 1 recombinant floe1 cloning - by Bioz Stars, 2026-10
    93/100 stars

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    Related Articles

    Recombinant:

    Article Title: CONFORMATIONAL ENSEMBLES OF INTRINSICALLY DISORDERED REGIONS AS MODULATORS OF TRANSCRIPTIONAL ACTIVITY
    Article Snippet: .. 4.2.2.1 Recombinant FLOE1 cloning, expression, purification, and phase separation FLOE1’s coding sequence was transferred from G18395 (see Plant plasmid construction section) into pDEST-HisMBP (Addgene plasmid # 11085) (Nallamsetty et al., 2005) using LR Clonase II (Thermo Fisher Scientific). .. A sequence encoding the Tobacco Etch Virus (TEV) protease recognition sequence, ENLYFQ, was then added between the MBP and FLOE1 coding sequences using the Q5 Site-Directed Mutagenesis Kit (New England Biolabs) with primers priTEVFWD/REV to generate pDEST-HisMBP-ENLYFQ-FLOE1.

    Cloning:

    Article Title: CONFORMATIONAL ENSEMBLES OF INTRINSICALLY DISORDERED REGIONS AS MODULATORS OF TRANSCRIPTIONAL ACTIVITY
    Article Snippet: .. 4.2.2.1 Recombinant FLOE1 cloning, expression, purification, and phase separation FLOE1’s coding sequence was transferred from G18395 (see Plant plasmid construction section) into pDEST-HisMBP (Addgene plasmid # 11085) (Nallamsetty et al., 2005) using LR Clonase II (Thermo Fisher Scientific). .. A sequence encoding the Tobacco Etch Virus (TEV) protease recognition sequence, ENLYFQ, was then added between the MBP and FLOE1 coding sequences using the Q5 Site-Directed Mutagenesis Kit (New England Biolabs) with primers priTEVFWD/REV to generate pDEST-HisMBP-ENLYFQ-FLOE1.

    Expressing:

    Article Title: CONFORMATIONAL ENSEMBLES OF INTRINSICALLY DISORDERED REGIONS AS MODULATORS OF TRANSCRIPTIONAL ACTIVITY
    Article Snippet: .. 4.2.2.1 Recombinant FLOE1 cloning, expression, purification, and phase separation FLOE1’s coding sequence was transferred from G18395 (see Plant plasmid construction section) into pDEST-HisMBP (Addgene plasmid # 11085) (Nallamsetty et al., 2005) using LR Clonase II (Thermo Fisher Scientific). .. A sequence encoding the Tobacco Etch Virus (TEV) protease recognition sequence, ENLYFQ, was then added between the MBP and FLOE1 coding sequences using the Q5 Site-Directed Mutagenesis Kit (New England Biolabs) with primers priTEVFWD/REV to generate pDEST-HisMBP-ENLYFQ-FLOE1.

    Purification:

    Article Title: CONFORMATIONAL ENSEMBLES OF INTRINSICALLY DISORDERED REGIONS AS MODULATORS OF TRANSCRIPTIONAL ACTIVITY
    Article Snippet: .. 4.2.2.1 Recombinant FLOE1 cloning, expression, purification, and phase separation FLOE1’s coding sequence was transferred from G18395 (see Plant plasmid construction section) into pDEST-HisMBP (Addgene plasmid # 11085) (Nallamsetty et al., 2005) using LR Clonase II (Thermo Fisher Scientific). .. A sequence encoding the Tobacco Etch Virus (TEV) protease recognition sequence, ENLYFQ, was then added between the MBP and FLOE1 coding sequences using the Q5 Site-Directed Mutagenesis Kit (New England Biolabs) with primers priTEVFWD/REV to generate pDEST-HisMBP-ENLYFQ-FLOE1.

    Sequencing:

    Article Title: CONFORMATIONAL ENSEMBLES OF INTRINSICALLY DISORDERED REGIONS AS MODULATORS OF TRANSCRIPTIONAL ACTIVITY
    Article Snippet: .. 4.2.2.1 Recombinant FLOE1 cloning, expression, purification, and phase separation FLOE1’s coding sequence was transferred from G18395 (see Plant plasmid construction section) into pDEST-HisMBP (Addgene plasmid # 11085) (Nallamsetty et al., 2005) using LR Clonase II (Thermo Fisher Scientific). .. A sequence encoding the Tobacco Etch Virus (TEV) protease recognition sequence, ENLYFQ, was then added between the MBP and FLOE1 coding sequences using the Q5 Site-Directed Mutagenesis Kit (New England Biolabs) with primers priTEVFWD/REV to generate pDEST-HisMBP-ENLYFQ-FLOE1.

    Plasmid Preparation:

    Article Title: CONFORMATIONAL ENSEMBLES OF INTRINSICALLY DISORDERED REGIONS AS MODULATORS OF TRANSCRIPTIONAL ACTIVITY
    Article Snippet: .. 4.2.2.1 Recombinant FLOE1 cloning, expression, purification, and phase separation FLOE1’s coding sequence was transferred from G18395 (see Plant plasmid construction section) into pDEST-HisMBP (Addgene plasmid # 11085) (Nallamsetty et al., 2005) using LR Clonase II (Thermo Fisher Scientific). .. A sequence encoding the Tobacco Etch Virus (TEV) protease recognition sequence, ENLYFQ, was then added between the MBP and FLOE1 coding sequences using the Q5 Site-Directed Mutagenesis Kit (New England Biolabs) with primers priTEVFWD/REV to generate pDEST-HisMBP-ENLYFQ-FLOE1.



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    4 2 2 1 recombinant floe1 cloning  (Addgene inc)
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    Addgene inc 4 2 2 1 recombinant floe1 cloning
    4 2 2 1 Recombinant Floe1 Cloning, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/4+2+2+1+recombinant+floe1+cloning/pGL3-Basic-9xSeq2-Luc+(Plasmid+%2342214)/flores_eduardo__2025__conformational_ensembles_of_intrinsically_disordered_regions_as_modulators_of_transcriptional_activity-1572-0-23
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    		  Buy from Supplier

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